We report on the calibration of gas sensors based on two transition metal dichalcogenides, molybdenum disulfide or tungsten diselenide, grown by the thermally assisted conversion of patterned Mo or W. The sensors showed non-stationary behavior when exposed to ammonia (NH₃) in the range of 10–100 parts per million at room temperature. This drawback hampered the calibration of the sensors. Applying the time-differential signal output (TDSO) enabled us to overcome the issue since the maxima of TDSO were uniquely and linearly correlated to the NH₃ concentration. The outcomes show that TDSO is a powerful, reliable, and valid approach when gas sensors are exposed to both oxidizing and reducing atmospheres.