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Authors:
Buchfellner, Andrea; Yurlova, Larisa; Nüske, Stefan; Scholz, Armin M.; Bogner, Jacqueline; Ruf, Benjamin; Zolghadr, Kourosh; Drexler, Sophie E.; Drexler, Guido A.; Girst, Stefanie; Greubel, Christoph; Reindl, Judith; Siebenwirth, Christian; Romer, Tina; Friedl, Anna A.; Rothbauer, Ulrich 
Document type:
Zeitschriftenartikel / Journal Article 
Title:
A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells 
Journal:
PLOS ONE 
Volume:
11 
Issue:
Year:
2016 
Pages from - to:
1-23 
Language:
Englisch 
Abstract:
Poly(ADP-ribose) polymerase 1 (PARP1) is a key player in DNA repair, genomic stability and cell survival and it emerges as a highly relevant target for cancer therapies. To deepen our understanding of PARP biology and mechanisms of action of PARP1-targeting anti-cancer compounds, we generated a novel PARP1-affinity reagent, active both in vitro and in live cells. This PARP1-biosensor is based on a PARP1-specific single-domain antibody fragment (~ 15 kDa), termed nanobody, which recognizes the N-...    »
 
ISSN:
1932-6203 
Article ID:
e0151041 
Department:
Fakultät für Luft- und Raumfahrttechnik 
Institute:
LRT 2 - Institut für Angewandte Physik und Messtechnik 
Chair:
Dollinger, Günther 
Open Access yes or no?:
Ja / Yes