The live cell irradiation and observation setup at SNAKE
Titel Sammlung:
Proceedings of the 11th International Conference on Nuclear Microprobe Technology and Applications and the 3rd International Workshop on Proton Beam Writing
Zeitschrift:
Nuclear Instruments and Methods in Physics Research Section B
Jahrgang:
267
Heftnummer:
12-13
Konferenztitel:
International Conference on Nuclear Microprobe Technology and Applications (11., 2008, Debrecen)
Tagungsort:
Debrecen
Jahr der Konferenz:
2008
Jahr:
2009
Seiten von - bis:
2090-2097
Sprache:
Englisch
Stichwörter:
Heavy ion cell irradiation ; Microbeam ; Live cell imaging ; Microscopy ; GFP
Abstract:
We describe a new setup at the ion microprobe SNAKE (Superconducting Nanoscope for Applied nuclear (Kern-) physics Experiments) at the Munich 14 MV Tandem accelerator that facilitates both living cell irradiation with sub micrometer resolution and online optical imaging of the cells before and after irradiation by state of the art phase contrast and fluorescence microscopy. The cells are kept at standard cell growth conditions at 37 °C in cell culture medium. After irradiation it is possible to switch from single ion irradiation conditions to cell observation within 0.5 s. First experiments were performed targeting substructures of a cell nucleus that were tagged by TexasRed labeled nucleotides incorporated in the cellular DNA by 55 MeV single carbon ion irradiation. In addition we show first online sequences of short time kinetics of Mdc1 protein accumulation in the vicinity of double strand breaks after carbon ion irradiation. «
We describe a new setup at the ion microprobe SNAKE (Superconducting Nanoscope for Applied nuclear (Kern-) physics Experiments) at the Munich 14 MV Tandem accelerator that facilitates both living cell irradiation with sub micrometer resolution and online optical imaging of the cells before and after irradiation by state of the art phase contrast and fluorescence microscopy. The cells are kept at standard cell growth conditions at 37 °C in cell culture medium. After irradiation it is possible to... »